METHODOLOGY DEVELOPMENT IN SOLID-STATE NMR OF RNA

Solid-state NMR is new emerging technique that can be applied to biomolecular complexes of any size. In our group we have pioneered application of solid-state NMR for RNA and protein-RNA complexes and have obtained first de novo structure of RNA by solid-state NMR approach. Currently we are developing advanced isotope labeling schemes and new experiments, both in conventional (13C-, 15N-detected) as well as 1H-detected ssNMR. We develop new experiments for paramagnetic relaxation enhancement (PRE) in ssNMR, study dynamics of RNA by ssNMR and design new strategies for Dynamic Nuclear Polarization (DNP) of protein-RNA complexes (in collaboration with university Frankfurt). Newly developed methods will open new possibilities for both NMR and structural biology and contribute to the fast-growing field of RNA therapeutics.